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Original subtitles

TOHO

TOHO COMPANY, LTD

Parasite Eve

Hiroshi Mikami

Riona Hazuki

Tomoko Nakajima

Ayako Omura

Goro Inakagi

Hisako Manda

Tetsuya Bessho

Original Novel by Hideaki Sena

Written by Ryoichi Kimizuka

Music by Joe Hisaishi

Directed by Masayuki Ochiai

Every organism we know is built up of cells.

Human beings, of course, are also masses of cells.

Our skin, bones, organs. . . They're all made of cells.

Each has a nucleus at its center.

Inside it . . .

Is the blueprint from which our body parts are made.

This is DNA, the genes.

Many other things are packed into the cell.

One part, like the nucleus, has its own DNA.

. . . The "mitochondrion".

The main function of these mitochondria. . .

. . .Is energy creation.

But why are these mitochondria here?

They have their own genes.

Thus, mitochondria were once organisms.

Parasites.

They are another life form that invaded our cells.

Parasitism

Mariko!

Now. . .

Here is something very strange. . .

Both parents pass on things like character or appearance.

Mitochondria is only passed through the mother.

The mitochondria in the bodies of everyone here. . .

All came from our mothers.

So in mitochondrial DNA. . .

We see the mother of us all.

We discover who the "Eve" of mythology was.

We go 200,000 years into the distant past.

We arrive in Africa.

It was here that "Eve" appeared.

The first human, the first woman to pass on mitochondria.

We call her "Mitochondria Eve".

Why did you stop coming?

I explained this. . .

You have to come three times a week. . .

And take out the things your body doesn't need.

I'm tired of it.

At this school. . .

We study the inside of the body to see how medicine works.

You have to wash your hands and gargle to keep germs out.

But the real fight against germs is inside you.

There are all sorts of tiny things living inside us.

You're lying!

Yuck!

I told you before about the immune system. . .

Excuse me. . .

I don't believe that!

You can only digest food. . .

Because you have bacteria in your intestines. . .

That's gross!

Hey! A snail!

Everyone! Shall I tell you a story?

What story?

About the bugs inside a snail.

Again?

They make the snail's shell hard.

So at first they're good for the snail.

With a hard shell it can live longer.

But when the snail gets big. . .

The bugs go up into its horns.

Why?

Usually. . .

Snails like dark places, but now they come into the sun.

The bugs make the horns swell. . .

And sway back and forth.

Why do they do that?

Only the birds in the sky know that.

The horns look like an insect that the birds love to eat.

Yes. . .

What the bugs really want. . .

Is to go far away in a bird's stomach.

They get the birds to eat the snail.

That's awful!

All right! That's all for science class today.

Time to go.

Are we all right?

We have lots of germs living inside us.

Will they control us when we get too big?

We'll be fine. We're a higher, more complicated life form.

Let's go.

We were parasitized as microbes a billion years ago.

But at some point they became vital to us.

We move, we think, because of mitochondria.

Both sides have chosen symbiosis.

Symbiosis

Sir!

Go ahead.

Uhh. . .

Is the study of these "mitochondria". . .

Of any possible use to anyone?

We now know that abnormalities in mitochondria DNA. . .

Often occur in conditions like diabetes and cirrhosis.

Illness can also be described as "energy loss".

Healthy mitochondria could be grown. . .

And inserted in diseased cells.

With cell function restored, the condition could be cured.

That's what I study.

Let's see some mitochondria.

Thank you.

Sorry. I can't come home yet. I've still got work to do.

It can't wait?

I have to get this data.

We're still working just with animals.

But soon we might be able to use mitochondria to help the sick!

Oh, that's right!

You forgot. . .

Our. . .

First. . .

Anniversary. . .

I bought you a present. . .

I feel belittled.

It's all right. I half-expected it.

I'm sorry.

See you later.

Don't look like that. . .

Work hard.

I'll leave as soon as I can.

I've waited so long for you. . .

What's wrong?

It's nothing.

How was the lecture, Doctor?

OK, I guess.

I tried this concentration.

Dr. Nagashima!

What did you use it for?

A gel dye.

All right.

Dr. Nagashima! The rat livers are improving!

The healthy cells we put in last week. . .

Have reversed the atrophy.

This is last week's.

Finally!

All right!

Culture those cells and check the mitochondria.

Right!

Nagashima. . . Don't work Ms. Asakura too hard.

She has a paper to present next month.

Yes, sir.

It's fine! I learn a lot by helping Dr. Nagashima!

I can understand your fascination.

There's so much we still don't know.

Excuse us!

Mitochondria were once. . .

Different life forms, weren't they. Like bacteria.

So what?

The children were asking me. . .

If one day they could take control of a human body.

There's a theory that claims. . .

Mitochondria doesn't have enough DNA to be independent.

Some of it was transferred over to the nucleus.

So that means some of the genes in the nucleus. . .

Used to belong to the mitochondria. . .

Well, yes, but. . .

Do your mitochondria have their own agenda?

Liver-Cell Culture

Dr. Nagashima!

Are you a family member?

How is she?

Badly injured.

Will she live?

Her breathing stopped from bleeding in the brain.

We're operating now.

Please inform the rest of her family.

Are you her husband?

Yes.

There was severe brain hemorrhage.

We operated as soon as she was brought in.

We put her on a respirator.

She's not breathing spontaneously.

We're trying heavy doses of stimulants.

There's loss of brain function. . .

The stem was also damaged.

What chance is there she'll regain consciousness?

I'm sorry to have to say. . . She is nearing brain death.

It's me. . .

Can you hear me?

Kiyomi. . .

You've got to wake up!

Today's a big day. . .

Our first. . .

Toshiaki!

Kiyomi. . .

It's too soon to follow your mother. . .

These are the brain function tests from last night.

These are from six hours later.

They are all negative. The brain is no longer functioning.

She has been pronounced brain-dead.

Mr. Nagashima. . .

This is Ms. Odagiri. She's our Transplant Coordinator.

Pleased to meet you.

Kiyomi had offered her kidneys for transplant.

Transplant?

Kiyomi was registered with the organ bank as a donor.

Were you aware?

She suddenly registered about six months ago.

Kiyomi's kidney could help. . .

A patient with chronic renal failure.

Unfortunately, there is no cure for this condition.

The patient must undergo regular dialysis.

Nagashima, sir. . .

I assure you, Kiyomi's kidney will not be wasted.

I think we should respect her wish.

If this is what Kiyomi wanted, we should allow it.

Are you Kiyomi's father?

Would you please sign this form of consent?

Mr. Nagashima!

Could we at least test for compatibility?

We have a potential kidney donor here in the city.

A woman, 25, brain-dead from hemorrhaging.

I compared HLA results for a suitable recipient

(HLA = Human Leuokocyte Antigen - Proteins that determine whether a patient will react against a donor transplant)

Your patient. . .

Leads the list.

I see.

There are no mismatches.

Shall I make the request?

Please.

Give me a little more time.

The family has yet to fully agree.

Which hospital is it?

Nurse. . .

Does this mean. . . Kiyomi is dead?

I've come to ask you to agree to the transplant.

The patient's HLA profile matches that of your wife.

Mr. Nagashima!

Mr. Nagashima, I understand how you must feel.

But this was her wish. The kidney will not go to waste.

Please, free this patient from her suffering.

She's a 12 year old girl.

My name is Yoshizumi, I'm with the Central Hospital.

Doctor! Please let me handle this!

Let us use the kidney.

Doctor, please! This is against the rules!

She's just a little girl!

I want to set her free, Mr. Nagashima!

Mr. Nagashima!

Doctor!

Just hear me out! Please.

Mr. Nagashima!

Give it to me! I beg of you!

I've already failed once.

I transplated a kidney into the girl two years ago.

I thought we had succeeded.

But rejection set in.

The girl trusted me.

I let her down.

Please!

Give me another chance!

Kiyomi's alive.

Kiyomi is. . .

Not dead.

She's still alive!

Liver-Cell Culture

I will agree to the transplant.

Really?

But. . .

On one condition. . .

In return for her kidney, you will give me her liver.

What for?

I'm not a medical doctor. I can't dissect her.

You can take her liver out for me.

But. . .

What will you do with it?

Mariko. . .

Will you trust me. . .

One more time?

Her heart has stopped.

11:31 A.M.

Death confirmed.

I was of no help. . .

We'll begin the operation.

Please.

Please. . .

What was that about?

Hurry up.

12:04 P.M.

33 minutes after clinical death.

Beginning extraction.

Merry Christmas!

Here. . .

Next!

Yes. . .

Two, please.

Here. . .

Two.

Today's my birthday. So give me two.

Oh, is that so?

Kataoka. . .

"Kiyomi"?

The same character used for "Christmas Eve".

Happy birthday.

Only one?

Give me a break. It's Christmas Eve!

What's the matter?

The students in the lab are giving me a hard time.

They say I should do something before you run away.

No comment.

I just might do that.

I've. . .

Waited so long for you. . .

So have I.

Ever since the first time. . .

I saw you. . .

Let's take them out.

What's wrong?

Nothing. . .

It's hot. . .

Your daughter will be fine.

We'll prep her now. The doctor will be here shortly.

Doctor. . .? What is it doctor?

Doctor!

What are you doing?!

Taking the liver.

We're not here for that!

It won't take long.

There's no time to waste! Stop what you're doing.

Be quiet!

Wait right here.

It's your wife's liver.

Mr. Nagashima!

What about your wife's body?

What's going on?

Would you mind leaving?

Get out of here!

Shouldn't you be with your wife?

Kiyomi is. . .

Right here.

Time since donor death: 123 minutes.

Time since extraction: 69 minutes.

Beginning transplant.

Time: 1:34 P.M.

The kidney. . .

Mariko. . .

She's being moved to a sterile room. Don't worry.

The operation went well.

Doctor!

This time she'll be fine.

Thank you very much!

You're so beautiful, Kiyomi . . .

Mariko hasn't looked that happy in years. . .

What should I do to thank the donor's family?

Doctor! It's Mariko!

Mariko!

What's wrong?!

She just had a bad dream.

It wasn't a dream!

Mariko it was just a dream!

I swear it wasn't a dream! It wasn't!

You're going to be fine!

The doctor's here!

Mariko, try to calm down. . . Mariko!

It's here!

It called out to something!

Inside of my stomach!

Doctor! Hurry! Take it out!

Hurry! Take it out!

Hurry! Get it out!

Mariko!

The operation was a success!

There's nothing to worry about, Mariko!

Mariko, be brave. . .

You'll be all right, Mariko!

All right, Kiyomi. . .

Come on. . .

These are the new kidney cells magnified 16,000 times.

What do you think?

I've never seen mitochondria like those.

The size of these mitochondria. . .

These are three times their normal size!

There's more of them, too.

And they're replicating. . .

Faster with each passing day.

We took this after the surgery. It looked strange even then.

You gave the donor's liver to her husband, didn't you?

What was that about?

What is it, Kiyomi?

Kiyomi!

To-shi-a-ki. . .

Kiyomi?

Could I talk to you about my paper. . .?

I'm busy.

Was there something about your wife that I didn't know?

The kidney's cells are replicating in a frenzy!

Mr. Nagashima.

What happened to the liver?

What did you do with the liver?!

Kiyomi is alive.

Did you. . .

Think I would just stand idle while she died?

You're not culturing those cells, are you?

Anyone else would have just had to watch her die.

But I've. . .

Found a way to keep Kiyomi alive.

What do you think you're doing?

Why are you. . Why are you culturing your wife's cells?!

Kiyomi is inside each and every one of those cells.

You're insane!

You. . .

Revived Kiyomi by transplanting her kidney. . .

Into your patient.

I am. . .

Reviving Kiyomi by culturing her cells.

What's the difference?!

"The enzyme stimulating peroxisome growth is taken-

from fatty unsaturated acids in the mitochondria."

"The mitochondria of rat livers. . ."

"The enzyme stimulating peroxisome growth is taken. . ."

"The enzyme stimulating peroxisome growth is taken-

from fatty unsaturated acids-

in the mitochondria of rat livers. . ."

"In the mitochondria of. . ."

"The enzyme stimulating peroxisome growth is taken. . ."

Eve: Liver-Cell Culturing

. . .The Kiyomi Preparation. . .

Kiyomi has spoken to me.

To-shi-a-ki. . .

Kiyomi?

Look at my body.

You remember it. . .

Don't you?

You held it close so many times. . .

You loved me. . .

And only me.

It's me.

Toshiaki. . .

I've waited so long for this moment. . .

For a billion years. . .

Give me your love. . .

A billion years' worth. . .

It's raining.

Still I hear "Toshiaki". . .

It was the same strange voice I heard when I first saw him.

Like ringing in the ears. . .

Again, something hot wells up inside me.

My veins feel like they might burst. . .

And I can't breathe. . .

The voice is pleased when he talks about his work.

The word "mitochondria" alone makes my heart race. . .

Violently. . . With such overwhelming joy. . .

I'm losing myself.

I will soon be. . .

Controlled by that voice. . .

By that proud voice. . .

The one who loved you was me.

Kiyomi loved you. . .

Because I made her.

Christmas Eve. . .

I made her speak to you that day.

In a voice you would love. . .

It took your breath away. . .

I chose you.

You are. . .

The one who could culture us.

Who are. . .

You. . .?

I. . .

Have been. . .

Waiting for you. . .

I'm scared!

Doctor. . .

I'll be presenting my paper now.

I have something to tell. . .

All of humanity.

I've been looking for you.

The girl is becoming stranger every day!

Her uterus is changing!

Her cell structure, too!

Thank you very much.

National Biochemistry University, Sachiko Asakura. . .

Will now speak on "Retinoid Receptors-

in the Derivation of Mitochondrial DNA".

You humans who hear us today are indeed fortunate.

You will be the first to hear of a world to begin anew.

And we, too. . .

Are pleased of the opportunity to address humanity.

At last. . .

Mitochondria will be free!

What is wrong with her?!

Mitchondria...

Are speaking. . .

We have known you since you were microorganisms

We have provided you the energy with which you have evolved.

We even remember your ancestor, "Mitchondria Eve".

Long have we waited. . .

For this moment. . .

A billion years.

You are. . .

Eve, aren't you?!

What are you doing? This isn't your topic. . .

Silence!

We. . .

Can summon the mitochondria in your cells. . .

The energy they emit is under our control.

Mitochondrial energy converted to heat. . .

Thousands of volts!

But. . .

Impossible!

We waited for humans to reach this stage in evolution.

You have exceeded our expectations. . .

But now your role is complete.

Now. . .

We. . .

Will take your place.

A new evolution will begin.

We have. . .

Found a womb that is ideal for our purposes.

Our descendants. . .

Will be the ultimate life form.

Unfortunately. . .

You will not exist to see them flourish.

You will. . .

Be annihilated.

Asakura!

She's all right.

What have I done?!

Mitochondria and I. . . ?

We're going to have a child.

But. . .

Just with sperm alone. . .

Mitochondria isn't capable of creating a child. . .

My patient's uterus. . .

Is transforming. . .

Get away!

What's wrong?!

No!

Mariko, what's wrong?!

Get away!

Mariko!

Stay away!

What is it?!

Stay away!

What's wrong, Mariko?!

Mariko!

I'm hot!

Where?

I'm hot!

What happened?

Kiyomi's mitchondria. . .

Has a will of their own.

The mitochondria. . .

Needs a suitable womb.

They made Kiyomi donate her kidney.

They planned everything!

Even my culturing of her cells.

What about my patient?!

Mariko!

This way!

Mariko!

It's here.

It's here?

What's here?

It's here!

Who are you?!

Stop this, Kiyomi!

Give me back my daughter!

Hold it!

Don't let her out of this building!

Doctor. . .

Doctor, help me.

Doctor, I'm here.

Mariko?

Doctor. . .

Doctor, I'm here. . .

Doctor. . .

Doctor, help me. . .

Doctor. . .

I'm here.

Are you OK?!

The roof. . .

Doctor!

My child grows inside of you.

Wait!

Let her go!

Give her back!

Give the girl back, Kiyomi!

Do not interfere.

This is evolution.

The child she bears will be the true Mitochondria Eve.

Kiyomi. . .

Are you gone. . .?

At long last she noticed us.

We've been waiting for her.

We made her faint. We caused the crash.

And as we thought. . .

You cultured her cells. . .

And now we have the perfect womb.

Farewell. . .

Mankind.

I can't forget.

Nor can we.

It was difficult to form the Kiyomi you would love.

The first day I saw her. . .

Christmas Eve.

The night you and Kiyomi met.

A fate we orchestrated.

We made the Kiyomi you loved.

I saw her. . .

Before that. . .

The day she started university. . .

On campus.

She wore a pink dress. She was so cute. . .

A bright, innocent smile. . .

I stared at her. . .

Thinking how nice life would be. . .

To be with her.

Kiyomi's heart raced for you. . .

Because we made it do so!

We created Kiyomi's love for you.

Kiyomi. . .

Stay away!

Come. . .

Stop!

Come. . .

Come with me. . .

Stop!

Stay away!

Let me. . .

Hold you!

Toshiaki. . .

What happened?

You were dreaming.

Two, please.

Today's my birthday.

So give me two.

Kataoka. . .

"Kiyomi"?

The same character used for "Christmas Eve".

Thanks Santa.