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PETER REDDIEN: What are our steps?
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So we have a pedigree and the first thing we want to do
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is try to determine the mode of inheritance for our trait.
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Autosomal recessive, autosomal dominant, sex-linked,
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and so on.
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We talked about that previously.
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Next, we want to ascribe genotypes
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to the individuals in the pedigree.
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We will do that for our disease gene
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alleles based on the phenotype and what we can infer.
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And for the SSRs, we'll do molecular tests
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as we talked about last time to identify
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the genotype for the SSRs.
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Then we want to look at the pedigree
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and identify what are called informative meioses.
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And I'll get into that in the second part
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of the lecture, what those are.
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Then we want to categorize the genotypes of any gametes we
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can infer that were generated in the pedigree
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as being recombinant or non-recombinant.
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So we want to identify any recombinant or non-recombinant
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gametes that were generated that produced the individuals
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in the pedigree.
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And then with that information, we
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will then calculate a LOD score.
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I defined this as a statistical test
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we'll be using at the end of last lecture
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that will allow us to get some information about linkage
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between our polymorphisms and our disease-carrying gene.
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So those are our steps.
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All right, so to be able to do this,
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we just need to think about--
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we've talked about this.
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We need to think about how you identify informative meioses
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and identify recombinant and non-recombinant gametes.
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And then with that information, how do we actually
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calculate the LOD score?
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